Hemoglobin synthesis in human BFU-E and CFU-E-derived erythroid colonies
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چکیده
منابع مشابه
Hemoglobin synthesis in human BFU-E and CFU-E-derived erythroid colonies.
We have analyzed the synthesis of fetal hemoglobin (HbF) in the differentiated erythroid colonies produced by erythropoietin-responsive committed erythroid progenitors in plasma cultures of human marrow and peripheral blood. Two independent biochemical techniques. carboxymethyl-cellulose column chromatography of 3H-leucine-Iabeled globin. and polyacrylamide gel electrophoresis of 59Fe-labeled h...
متن کاملFetal hemoglobin in mixed hemopoietic colonies (CFU-GEMM), erythroid bursts (BFU-E) and erythroid colonies (CFU-E): assessment by radioimmune assay and immunofluorescence.
Colony assays are now available to study erythroid differentiation at three different levels. Mixed hemopoietic colonies represent progeny of pluripotent progenitors (CFU-GEMM). Erythroid bursts and cobnies are derived from early (BFU-E) and late precursors (CFU-E) that are committed towards erythropoiesis. The three different types of colonies were examined for their content of fetal hemoglobi...
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Bone marrow erythroid progenitor cells were examined from 50 cases of acute leukaemia and from 20 normal subjects using an in vitro semisolid culture method. Numbers of both primitive erythroid progenitor cells (BFU-e) and later-stage erythroid progenitor cells (CFU-e) were remarkably depressed in patient with acute leukaemia in active phase. However, both BFU-e and CFU-e recovered to within no...
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Erythroid progenitors (B-8, B-4, CFU-e) in the femoral marrow of polycythemic mice were measured by in vitro culture assays after a single administration of BCNU or Myleran. BCNU reduced pluripotent stem cells to 40% and erythroid progenitors to less than 5% of normal. B-8, the earliest erythroid progenitors, regenerated without erythropoietin (Epo) completely within 5 days. At 14 days after BC...
متن کاملGeneration of committed erythroid BFU-E and CFU-E progenitors does not require erythropoietin or the erythropoietin receptor
Erythropoietin (EPO) is the principal growth factor regulating the production of circulating erythrocytes. We introduced null mutations into both Epo and the EPO receptor (EpoR) gene. Both heterozygotes appeared normal. Homozygous animals exhibited reduced primitive erythropoiesis and died around embryonic day 13, owing to failure of definitive fetal liver erythropoiesis. Both types of mutation...
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ژورنال
عنوان ژورنال: Blood
سال: 1979
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v54.4.805.bloodjournal544805